Stéphane Dupas

dupas

Research scientist IRD
Member of : DEEIT

Fax 33 1 69 82 37 36
Mail. Stephane.Dupas(at)legs.cnrs-gif.fr

The ecological and evolutionary trajectories of biodiversity

The aim of my research is to use population genetic data to infer scenario on the dynamics of biodiversity at different time and spatial scales. I used different approaches detailed below. The most recent is bayesian inference of coalescence probabilities in small parameter population graph models. Graph representation is comon to many disciplines and can serve as boundary concept to link of dynamics of biodiversity to climatic scenario, or social networks. I work mostly on insects as model systems.


E
nvironmental demo-genetics
The population genetic data provide information on the spatial environmental and ecological dynamics
of species. The formalization and inference of the models from these environmental, genetic and social data can help to establish more integrative scenarios to predict the response of biodiversity to global change. But existing population genetics models often imply ideal homogeneous populations or limited number of populations and do not formalize relations with the heterogeneous environment in complex natural connectivity networks. My project is to infer such models in a Bayesian, graph theory and coalescence theory framework using functions linking environmental data and structures of social networks to the dynamics of genes. We work in the context of biological control operations where we adjust Markov dynamic process, and in the context of spatial and environmental dynamics of insect pests in Kenya and South America where we adjust graph theory metrics and coalescence Bayesian models.

Coevolutionary trajectories
Characterising genetic and phylogenetic systems shaping species interactions can help to better understand and predict their co-evolutionary trajectories. The genetic systems governing host parasitoid interaction (between fruit flies and parasitoid wasps of the genus Leptopilina Figitidae) and the selective forces estimated in coevolutionary experiments explain geographical patterns observed in Africa. These genetic systems also determine the long-term coevoluitonary trajectories. Nonspecific virulence systems have high phylogenetic inertia whereas more specific virulence systems lead to arms races with the host. Similar resistance – virulence patterns have been characterized in the case of the biological control agent against lepidopteran cereal stemborer in sub-Saharan Africa, the parasitoid Cotesia sesamiae (Hymenoptera: Braconidae). Geographic patterns in resistance virulence traits and in molecular neutral (microsatellites) and selected markers related to adaptation. Selected markers are associated to a polydnavirus intergrated in the wasp chromosomes, injected into lepidopteran host during the egg laying, expressed in the cells thereof and responsible for immune suppression and regulation host development for the benefit of the parasitoid. ABC Papogen project aims to study the genetic mechanisms of these adaptations through classical genetic studies and high throughput sequencing, either in the long term between locally adapted populations, or in the short term in the context of biological control or for the genetic selection of strains to be released.

 

 

Publications

  1. Petit, C., B. Le Ru, S. Dupas, B. Frerot, P. Ahuya, L. Kaiser-Arnauld, M. Harry & P.-A. Calatayud (2015) Influence of Dietary Experience on the Induction of Preference of Adult Moths and Larvae for a New Olfactory Cue. Plos One, 10.
  2. Kaiser, L., B. P. Le Ru, F. Kaoula, C. Paillusson, C. Capdevielle-Dulac, J. O. Obonyo, E. A. Herniou, S. Jancek, A. Branca, P.-A. Calatayud, J.-F. Silvain & S. Dupas (2015) Ongoing ecological speciation in Cotesia sesamiae, a biological control agent of cereal stem borers. Evolutionary Applications, 8, 807-820.
  3. Arca, M., F. Mougel, T. Guillemaud, S. Dupas, Q. Rome, A. Perrard, F. Muller, A. Fossoud, C. Capdevielle-Dulac, M. Torres-Leguizamon, X. X. Chen, J. L. Tan, C. Jung, C. Villemant, G. Arnold & J. F. Silvain (2015) Reconstructing the invasion and the demographic history of the yellow-legged hornet, Vespa velutina, in Europe. Biological Invasions, 17, 2357-2371.

  4. Dupas S, Le Ru B, Branca a et al. (2014) Phylogeography in continuous space: coupling species distribution models and circuit theory to assess the effect of contiguous migration at different climatic periods on genetic differentiation in Busseola fusca (Lepidoptera: Noctuidae). Molecular ecology, 33, 1–13.

  5. Rebaudo, F., Le Rouzic, A., Dupas, S., Silvain, J.-F., Harry, M. & Dangles, O. (2013). SimAdapt: an individual-based genetic model for simulating landscape management impacts on populations. Methods in Ecology and Evolution, 4, 595–600.

  6. Dupas S., Poirié M., Frey F., Carton Y. 2013. Is parasitoid virulence against multiple hosts adaptive or constrained by phylogeny ? The study of Leptopilina spp. (Hymenoptera: Figitidae) / Drosophila (Diptera: Drosophilidae) interactions. Annales de la société entomologique de France.

  7. Jancek S., Bézier A., Gayral P., Paillusson C., Kaiser L., Dupas S., Le Ru B.P., Barbe V., Periquet G., Drezen J.-M., Herniou E.A. 2013. Adaptive selection on bracovirus genomes drives the specialization of Cotesia. Plos One.

  8. Carpio C., Dangles O., Dupas S., Léry X., López-Ferber M., Orbe K., Páez D., Rebaudo F., Santillán A., Yangari B., Zeddam JL. 2013. Development of a viral biopesticide for the control of the Guatemala potato tuber moth Tecia solanivora. J Invertebr Pathol. 112: 184-91.

  9. Sezonlin, M., Ndemah, R., Goergen, G., Le Rü, B., Dupas S., & Silvain, J.-F., 2012. Genetic structure and origin of Busseola fusca populations in Cameroon. Entomologia Experimentalis et Applicata, 145: 143-152

  10. Ndemah R., SchulthessF., Abang A., Ghogomu T., Ntonifor N. Dupas S., Le Ru B., 2012. Suitability of noctuid cereal stemborers in Cameroon to three Kenyan strains of the braconid larval parasitoid Cotesia sesamiae. Journal of Applied Entomology, 136 (1-2): 60-69.

  11. Serbielle C., Dupas S., Perdereau E., Ericourt F., Dupuy C., Huguet E., Drezen J. M. Evolutionary mechanisms driving the evolution of a large polydnavirus gene family coding for protein tyrosine phosphatases. BMC Evolutionary Biology, 2012, 12, p. 253.

  12. Branca, A., Dupas, S., Gitau, C. W. (2011). Maintenance of specialized parasitoid populations by polydnaviruses. In J.-M. Drezen & N. E. Beckage (Eds.), Parasitoid Viruses: Symbionts and Pathogens. Elsevier. Accepted for publication.

  13. Branca, A., Le Rü, B. P., Vavre, F., Silvain, J.-F., & Dupas, Stéphane. (2011). Intraspecific specialization of the generalist parasitoid Cotesia sesamiae revealed by polyDNAvirus polymorphism and associated with different Wolbachia infection. Molecular ecology, 959-971.

  14. Torres-Leguizamón, M., Dupas, S., Dardon, D., Gómez, Y., Niño, L., Carnero, a, et al. (2011). Inferring native range and invasion scenarios with mitochondrial DNA: the case of T. solanivora successive north–south step-wise introductions across Central and South America. Biological Invasions. 7: 1505-1519.

  15. Calatayud P.-A., Gitau C., Calatayud S., Dupas S., Le Rü B. & Silvain J.-F. (2011). Variability in the reproductive biology and in resistance against Cotesia sesamiae among two Busseola fusca populations. Journal of Applied Entomology 135: 423-429.

  16. Mailafiya D., Le Rü B., Kairu E.W., Calatayud Paul-André, et Dupas S. 2010. Factors affecting stem borer parasitoid species diversity and parasitism in cultivated and natural habitats. Environmental Entomology, 2010, 39 (1), p. 57-67.

  17. Hernandez N., Barragan, A, Dupas, S, Silvain JF, Dangles O 2010. Wing shape variations in an invasive moth are related to sexual dimorphism and altitude. Bulletin of Entomological Research.In press.

  18. Gitau Wanjiru C., Schulthess, F.. et Dupas S. 2010 : An association between host acceptance and virulence status of different populations of Cotesia sesamiae, a braconid larval parasitoid of lepidopteran cereal stemborers in Kenya. Biological Control. 54,100-106.

  19. Dupas S., Dubuffet A., Carton Y. & Poirié M. 2009. Local, Geographic and Phylogenetic Scales of Coevolution in Drosophila-Parasitoid Interactions. Advances in Parasitology, 70, 282-296

  20. Dubuffet A., Colinet D., Anselme C., Dupas S., Carton Y. & Poirié M. 2009. Variation of Leptopilina boulardi Success in Drosophila Hosts: What is Inside the Black Box? Advances in Parasitology, 70, 148-188.

  21. Branca, A., F. Vavre, J.-F. Silvain, and S. Dupas. 2009. Maintenance of adaptive differentiation by Wolbachia induced bidirectional cytoplasmic incompatibility: the importance of sib-mating and genetic systems. BMC Evol Biol., 9, 185.

  22. Faure N., Branca, A., Gigot, G., Dupas, S., Silvain, J-F. 2009. Isolation and characterization of ten microsatellite loci in Cotesia sesamiae (Cameron) (Hymenoptera: Braconidae), a parasitoid of African stem borer pests. Molecular Ecology Resources,9, 1375-1379.

  23. Mailafiya D, Rü BL, Kairu EW, Calatayud P-A, Dupas S. 2009. Species diversity of lepidopteran stem borer parasitoids in cultivated and natural habitats in Kenya. Journal of Applied Entomology, 133, 416-429.

  24. Torres-Leguimazon, M., Solignac, M., Vautrin, D., Capdevielle-Dulac, C., Dupas, S., Slvain, J.F. 2009. Isolation and characterization of polymorphic microsatllites in the potato tuber moth Tecia solanivora (Povolny, 1973) (Lepodoptera: Gelechiidae). Molecular Ecology Resources, 9, 1167-1169.

  25. Gitau C. W., Gundersen-Rindal D., Pedroni M., Dupas S., Mbugi J.-P. 2009 : Differential expression of the CrV1 haemocyte inactivation-associated polydnavirus gene in the african maize stemborer larvae parasitized by two biotypes of the endoparasitoid Cotesia sesamiae (Cameron). Infection Genetics and Evolution, 9 (3), p. 377-378.

  26. Zeddam, J.-L., Orbe, K., Léry, X., Dangles, O., Dupas, S., Silvain, J.-F. 2008. An isometric virus of the potato tuber moth Tecia solanivora (Povolny) (Lepidoptera: Gelechiidae) has a tri-segmented RNA genome. Journal of invertebrate Pathology. 99:204-211.

  27. Dupas, S., C.W. Gitau, A. Branca, B.P. Le Rü and J.-F. Silvain. Evolution of a polydnavirus gene in relation parasitoid host species immune resistance. The Journal of Heredity. 99: 491-499.

  28. Serbielle, C., S. Chowdhury, S. Pichon, S. Dupas, et al. 2008. “Viral cystatin evolution and three-dimensional structure modelling: A case of directional selection acting on a viral protein involved in a host-parasitoid interaction.” BMC Biology 6: 38.

  29. Zeddam, J-L., Orbe, K., Léry, X., Dangles, O., Dupas, S.; Silvain, J-F. 2008. An isometric virus of the potato tuber moth Tecia solanivora (Povolny) (Lepidoptera : Gelechiidae) has a tri-segmented RNA genome Journal of Invertebrate Pathology

  30. Almeida, C., R. Pacheco, K. Haag, S. Dupas, E. Dotson and J. Costa. 2008. Inferring from Cyt B gene the Triatoma brasiliensis Neiva, 1911 (Hemiptera : Reduviidae : Triatominae) genetic structure and domiciliary infestation in the state of Paraiba, Brazil. American Journal of Tropical Medicine & Hygiene. 78, 791–802.

  31. Calatayud, P.-A., Juma, G., Njagi, P. G. N., Faure, N., Dupas, S., Le Rü, B., Magoma, G., Silvain J.-F., Frerot, B. 2008. Differences in mate acceptance and host plant recognition between wild and laboratory-reared Busseola fusca (Fuller). J. Appl. Entomol.: 132, 255-264.

  32. Puillandre N., S. Dupas, O. Dangles, J.-L. Zeddam, C. Capdevielle Dulac, K. Barbin, M. Torres-Leguizamon and J.-F. Silvain (2008) Genetic bottleneck in invasive species : the potato tuber moth adds to the list. Biological Invasions 10:319-333.

  33. Gitau, C.W, D. Gundersen-Rindal, M. Pedroni, P.J. Mbugi and S. Dupas. 2007. Differential expression of the CrV1 haemocyte inactivation-associated polydnavirus gene in the African maize stem borer Busseola fusca (Fuller) parasitized by two biotypes of the endoparasitoid Cotesia sesamiae (Cameron). J. Insect Physiol. 57:676-684.

  34. Le Rouzic, A., S. Dupas, P. Capy, 2007 Genome ecosystem and transposable elements species. Gene 390: 214-220.

  35. Dubuffet, A., S. Dupas, F. Frey, J.-M. Drezen, M. Poirie and Y. Carton, 2007. Genetic interactions between the parasitoid wasp Leptopilina boulardi and its Drosophila hosts. Heredity 98: 21-27.

  36. Rincon, C., D.Bordat, B. Lörh, and S. Dupas, (2006). Reproductive isolation and differentiation between five populations of Cotesia plutellae (Hymenoptera; Braconidae), parasitoid of Plutella xylostella (Lepidoptera: Plutellidae ) Biological Control 36:171-182.

  37. Dupas, S., C.W. Gitau, B.P. Le Rü, N. Faure, P. Le Gall and J.-F. Sivain, 2006. Single-step PCR diff erentiation of Cotesia sesamiae (Cameron) and Cotesia fl avipes Cameron (Hymenoptera: Braconidae) using polydnavirus markers. Ann. Soc. Entomol.Fr. 42:319-323.

  38. Branca, A. and S. Dupas. 2006. A model for the study of Wolbachia pipientis Hertig (Rickettsiales: Rickettsiaceae)- induced cytoplasmic incompatibility in arrhenotokous haplodiploid populations: consequences for biological control. Ann. Soc. Entomol.Fr. 42:443-448.

  39. Gitau, C.W., S. Dupas, A.J. Ngi-Song, J.P. Mbugi and F. Schulthess 2006. Calyx fluid proteins of two Cotesia sesamiae (Cameron) (Hymenoptera: Braconidae) biotypes in Kenya: implications to biological control of the stem borer Busseola fusca (Fuller) (Lepidoptera: Noctuidae). Ann. Soc. Entomol.Fr. 42:433-441

  40. Sezonlin, M., S. Dupas, B.P. Le Rü, N. Faure, P. Le Gall and J.-F. Silvain 2006. Phylogeographic pattern and regional evolutionary history of the maize stalk borer Busseola fusca (Fuller) (Lepidoptera: Noctuidae) in sub-Saharan Africa. Ann. Soc. Entomol.Fr. 42:339-351.

  41. Ong’amo, G.O., Le Rü, B.P., Dupas, S., Moyal, P., Muchugu, E., Calatayud, P.-A. and J.-F. Silvain 2006. The role of wild host plants in the abundance of lepidopteran stem borers along altitudinal gradient in Kenya. Ann. Soc. Entomol.Fr. 42:363-370.

  42. Ong’amo, G. O., Le Rü, B., Dupas, S., Moyal, P., Calatayud, P. A. and Silvain, J.-F. 2006. Distribution, pest status and agroclimatic preferences of lepidopteran stemborers of maize and sorghum in Kenya. Annales de la Société Entomologique de France 42: 171-177.

  43. Otieno, N.A., B.P., Le Rü, G.O., Ong’amo, S. Dupas, P.-A. Calatayud, M., Makobe, J., Ochora and J.-F. Silvain, (2006). Diversity and abundance of wild host plants of lepidopteran stem borers in two diff erent agroecological zones of Kenya. Ann. Soc. Entomol.Fr. 42:371-380.

  44. Sezonlin, M., S.Dupas, B.Le Rü, P.Le Gall, P.Moyal, P.-A. Calatayud, I. Giffard, N. Faure, N. and J.-F. Silvain 2005. Phylogeography and population genetics of the maize stalk borer Busseola fusca (Lepidoptera, Noctuidae) in subsaharan Africa. Molecular Ecology 15:407-420.

  45. Dupas, S., Morand, S. & P. Eslin. 2004. Evolution of hemocyte concentration in the melanogaster subgroup species. C.R. Acad. Sci. Biologie, 327(2):139-47.

  46. Dupas, S., Turnbull, M.W. & B.A. Webb. 2003. Diversifying selection in a parasitoid’s symbiotic virus among genes involved in inhibiting host immunity. Immunogenetics, 55(6): 351-361.

  47. Dupas, S., Carton, Y. & Poirié, M 2003. The genetic dimension of the coevolution of virulence resistance in Drosophila – parasitoid wasp relationships. Heredity, 90(1): 84-89

  48. Dupas, S. & Boscaro, M. 1999. Geographic variations and evolution of immune suppressive genes in a Drosophila parasitoid. Ecography 22: 284-291.

  49. Dupas, S., & Carton, Y. 1999. Two non linked genes for specific virulence of Leptopilina boulardi against Drosophila melanogaster and D. yakuba. Evol. Ecol. 13: 211-220

  50. Dupas, S., Frey, F., & Carton, Y. 1998. A single parasitoid segregating factor controls immune suppression in Drosophila. J. of Heredity, 89: 306-311.

  51. Carton, Y. & Dupas, S. 1998. Genetic of resistance and virulence in host parasioid system IN “Laboratory manual on techniques in Insect Immunology” A. Wiesner, AG. Dunphy, V.J. marmaras, I. Morishima, M. Sugumaran and M. Yamakawa ed, SOS publications, Fair Haven, NJ chapter 33: 279-288

  52. Russo, J., Dupas, S., Frey, F., Brehelin, M., Carton, Y. 1996. Evolution of parasitoid egg surface during parasitization of Drosophila. Parasitology.