Archives
Joseph, Guilliet
Jonathan, Filée
Permanent Researcher (CR1), CNRS.
Equipe Elements Génétiques Mobiles (ELEGEM)
Tèl. 33 1 69 82 37 05
Fax 33 1 69 82 37 36
Jonathan.Filee@legs.cnrs-gif.fr
Evolutionnary genomics of Viruses and Transposons
Viruses are ubiquitous components of the biosphere and their number exceeds that of cells by at least an order of magnitude. This abundance is also accompanied by an extraordinary diversity in genome size, composition and organisation and viruses have been divided into many different classes based on these criteria. These observations raise multiple questions about the origin and evolution of different viral classes.
My researches focus on the diversity and genome evolution of various classes of mobile elements including large DNA viruses and phages, Retroviruses/Retrotransposons and DNA transposons. I’m also interested by the evolutionary relationships between these elements and their cellular hosts i.e. lateral gene exchange and exaptation of viral genes to accomplish cellular processes.
Curriculum vitae
2000 : DEA « Biodiversité : Génétique, Histoire et Mécanisme de l’évolution », Université Paris XI Orsay (France).
2000-2003 : PhD degree, Université Paris XI Orsay (France), IGM (Patrick Forterre Lab).
2003-2007 : Postdoc, Université Toulouse III (France), LMGM (Henry Krisch and Mick Chandler Labs).
2007- : Permanent Researcher (CR), CNRS, LEGS
Publications
Disdero, E. & J. Filée (2017) LoRTE: Detecting transposon-induced genomic variants using low coverage PacBio long read sequences. Mobile DNA, 8, 6. 10.1186/s13100-017-0088-x.
Filée J, Rouault JD, Harry M, Hua-Van A (2015) Mariner transposons are sailing in the genome of the blood-sucking bug Rhodnius prolixus. BMC genomics. 15;16(1):1061
Filée J (2015). Genomic comparison of closely related Giant Viruses supports an accordion-like model of evolution. Front Microbiol. 16;6:593
Filée J. (2014) Multiple occurrences of Giant Virus core genes acquired by Eukaryotic genomes: the visible part of the iceberg ? Virology. ;466-467:53-9.
Kamoun C, Payen T, Hua-Van A, Filée J. (2013) Improving prokaryotic transposable elements identification using a combination of de novo and profile HMM methods. BMC Genomics 11;14:700
Filée J. (2013) Route of NCLDV evolution: the genomic accordion. Curr Opin Virol. 3(5):595-9
Metcalfe CJ, Filée J, Germon I, Joss J, Casane D. (2012) Mode and tempo in inflation of size of the Australian lungfish (Neoceratodus forsteri) genome: a major role for CR1-like elements. Mol. Biol. Evol. 9(11):3529-39
Hua-Van A, Le Rouzic A, Boutin TS, Filée J, Capy P. (2011) The struggle for life of the genome’s selfish architects. Biol. Direct 6:19..
Filée J, Chandler M. (2010) Gene exchange and the origin of Giant Viruses. Intervirology 53(5):354-61.
Rouault JD, Casse N, Chénais B, Hua-Van A, Filée J, Capy P (2009) Automatic classification within families of transposable elements: application to the mariner Family. Gene.15;448(2):227-32.
Filée J (2009) Lateral gene transfer, lineage-specific gene expansion and the evolution of Nucleo Cytoplasmic Large DNA viruses.J Invertebr Pathol. 101(3):169-71
Filée J, Pouget N, Chandler M. (2008) Phylogenetic evidence for extensive lateral acquisition of cellular genes by Nucleocytoplasmic Large DNA Viruses.BMC Evol Biol. 26;8:320
Filee J, Siguier P, Chandler M (2007) Insertion Sequence diversity in Archaea. Microbiol Mol Biol Rev. 71(1):121-57
Filee J, Siguier P, Chandler M (2007) I am what I eat and I eat what I am: acquisition of bacterial genes by Giant Viruses. Trends Genet. 23(1):10-5
Siguier P, Filee J, Chandler M (2006) Insertion Sequences in Prokaryotic Genomes. Curr. Opin. Microbiol. 9(5):526-31
Filee J, Bapteste E, Susko E, Krisch HM (2006) A Selective Barrier to Horizontal Gene Transfer in the T4-Type Bacteriophages that Has Preserved a Core Genome with the Viral Replication and Structural Genes. Mol. Biol. Evol. 23(9):1688-1696
Filee J, Comeau AM, Suttle CA, Krisch HM (2006) T4-type bacteriophages. Med Sci. 22(2):111-2.
Filee J, Tetart F, Suttle CA, Krisch HM (2005) Marine T4-type bacteriophages, a ubiquitous component of the dark Matter of the biosphere. Proc.Natl.Acad.Sci.USA. 102(35):12471-6
Filee J, Forterre P (2005) Viral proteins functioning in organelles: a cryptic origin? Trends Microbiol. 13(11):510-3
Filee J, Forterre P, Laurent J (2003) The role played by viruses on the Evolution of their cellular host: a view on informational proteins phylogenies. Res. Microbiol. 154:237-43
Myllykallio H, Leduc D, Filee J, Liebl U (2003) Life without dihydrofolate reductase FolA. Trends Microbiol. 11(5):220-3
Gadelle D, Filee J, Buhler C, Forterre P (2003) Phylogenomics of type II DNA topoisomerases. BioEssay 25: 232-242.
Myllykallio H, Lipowsky G, Leduc D, Filee J, Forterre P, Liebl U (2002) An alternative flavin-dependent mechanism for thymidylate synthesis. Science 5578:105-7.
Filee J, Forterre P, Sen-Lin T, Laurent J (2002) Evolution of DNA polymerase families: evidences for multiple gene exchange between cellular and viral proteins. J Mol Evol 54:763-73.
Nicolas Pollet
Member of : Polygnome
Tel. 33 1 69 82 37 10
Fax 33 1 69 82 37 36
Nicolas.Pollet@egce.cnrs-gif.fr
Research themes
Genomics – Amphibians – Evolution – Transposon – Xenopus – Bioinformatics – Development
Publications
2021
- Gut microbial ecology of Xenopus tadpoles across life stages. Scalvenzi, T., Clavereau, I., Bourge, M. and Pollet, N. (2021) bioRxiv, 2020.05.25.110734, ver. 4 peer reviewed and recommended by Peer community in Genomics. https://doi.org/10.1101/2020.05.25.110734 https://doi.org/10.24072/pci.genomics.100005
- Transcriptomic analysis of the trade-off between endurance and burst-performance in the frog Xenopus allofraseri. Ducret, V., Richards, A.J., Videlier, M., Scalvenzi, T., Moore, K.A., Paszkiewicz, K., Bonneaud, C., Pollet, N. and Herrel, A. (2021) BMC Genomics 22(1):204. doi: 10.1186/s12864-021-07517-1.
- Draft nuclear genome and complete mitogenome of the Mediterranean corn borer, Sesamia nonagrioides, a major pest of maize. Muller, H., Ogereau, D., Da-Lage, J.L., Capdevielle, C., Pollet, N., Fortuna, T., Jeannette, R., Kaiser, L. and Gilbert, C. (2021) doi: 10.1093/g3journal/jkab155 G3 (Bethesda). 11(7):jkab155.
2020
- A new method for long-read sequencing of animal mitochondrial genomes: application to the identification of equine mitochondrial DNA variants. Dhorne-Pollet, S., Barrey, E. and Pollet, N. (2020) BMC Genomics 21(1):785. doi: 10.1186/s12864-020-07183-9.
- Evolutionary Dynamics of the Repetitive DNA in the Karyotypes of Pipa carvalhoi and Xenopus tropicalis (Anura, Pipidae). (2020) Front Genet. 11:637. doi: 10.3389/fgene.2020.00637. eCollection 2020.
- Ancient Adaptive Lateral Gene Transfers in the Symbiotic Opalina-Blastocystis Stramenopile Lineage.Mol Biol Evol. 37(3):651-659. doi: 10.1093/molbev/msz250.
2017
- Bronchain, O. J., A. Chesneau, A. H. Monsoro-Burq, P. Jolivet, E. Paillard, T. S. Scanlan, B. A. Demeneix, L. M. Sachs & N. Pollet (2017) Implication of thyroid hormone signaling in neural crest cells migration: Evidence from thyroid hormone receptor beta knockdown and NH3 antagonist studies. Molecular and Cellular Endocrinology, 439, 233-246. 10.1016/j.mce.2016.09.007.
- Maharana, S. K., N. Pollet & G. Schlosser (2017) Identification of novel cis-regulatory elements of Eya1 in Xenopus laevis using BAC recombineering. Scientific Reports, 7, 11. 10.1038/s41598-017-15153-7.
- Spirhanzlova, P., S. Dhorne-Pollet, J. S. Fellah, C. Da Silva, T. Tlapakova, K. Labadie, J. Weissenbach, J. Poulain, T. Jaffredo, P. Wincker, V. Krylov & N. Pollet (2017) Construction and characterization of a BAC library for functional genomics in Xenopus tropicalis. Developmental Biology, 426, 255-260. 10.1016/j.ydbio.2016.05.015.
2016
- Tissier, J., J. C. Rage, R. Boistel, V. Fernandez, N. Pollet, G. Garcia & M. Laurin (2016) Synchrotron analysis of a ‘mummified’ salamander (Vertebrata: Caudata) from the Eocene of Quercy, France. Zoological Journal of the Linnean Society, 177, 147-164.
2015
- Vouillot, L., Thélie, A. and Pollet N. (2015) Comparison of T7E1 and Surveyor Mismatch Cleavage Assays To Detect Mutations Triggered by Engineered Nucleases. G3 (Bethesda). doi: 10.1534/g3.114.015834.
- Colombo, B.M., Scalvenzi, T., Benlamara, S. and Pollet, N. (2015) Microbiota and mucosal immunity in amphibians. Front Immunol. 6:111. doi: 10.3389/fimmu.2015.00111.
- Tissier, J., Rage, J.C., Boistel, R., Fernandez, V., Pollet, N., G Garcia, G. and Laurin, M. (2015) Synchrotron analysis of a ‘mummified’ salamander (Vertebrata: Caudata) from the Eocene of Quercy, France. Zoological Journal of the Linnean Society in press doi: 10.1111/zoj.12341.
2014
- Plouhinec, J.L., Roche, D.D., Pegoraro, C., Figueiredo, A.L., Maczkowiak, F., Brunet, L.J., Milet, C., Vert, J.P., Pollet, N., Harland, R.M., Monsoro-Burq, A.H. (2014) Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers. Dev Biol. 386:461-72.
- Scalvenzi, T. and Pollet, N. (2014) Insights on genome size evolution from a miniature inverted repeat transposon driving a satellite DNA. Mol. Phyl. Evol. 81:1-9
2013
- Boistel, R., Aubin, T., Cloetens, P., Peyrin, F., Scotti, T., Herzog, P., Gerlach, J., Pollet, , Aubry, J.F. (2013) How minute sooglossid frogs hear without a middle ear. Proc Natl Acad Sci U S A 110:15360-4.
- Dhorne-Pollet, S., Thélie, A., Pollet, N. (2013) Validation of novel reference genes for RT-qPCR studies of gene expression in Xenopus tropicalis during embryonic and post-embryonic development. Dev Dyn. 242:709-17.
2012
- Sinzelle, L., Thuret, R., Hwang, H.Y., Herszberg, B., Paillard, E., Bronchain, O.J., Stemple, D.L., Dhorne-Pollet, S. and Pollet, N. (2012) Characterization of a novel Xenopus tropicalis cell line as a model for in vitro Genesis 50:316-24.
- Planson, A.G., Carbonell, P., Paillard, E., Pollet, N. and Faulon, J.L. (2012) Compound toxicity screening and structure-activity relationship modeling in Escherichia coli. Biotechnol Bioeng 109:846-50 doi : 10.1002/bit.24356
- Parain, K., Mazurier, N., Bronchain, O.J., Borday, C., Cabochette, P., Chesneau, A., Colozza, G., el Yakoubi, W., Hamdache, J., Gilchrist, M., Pollet, N. and Perron, M. (2012). A large scale screen for neural stem cell markers in Xenopus retina. Developmental Neurobiology. 72:491-506.
- Tempel, S., Pollet, N. and Tahi, F. (2012) ncRNAclassifier: a tool for detection and classification of transposable element sequences in RNA hairpins. BMC Bioinformatics. 13:246.
2011
- Demattei, M-V., Hedhili, S., Sinzelle, L., Bressac, C., Casteret, S., Moiré, N., Cambefort, J., Thomas, X., Pollet, N., Gantet, P., and Bigot, Y. (2011) Nuclear Importation of Mariner Transposases among Eukaryotes : Motif Requirements and Homo-Protein Interactions. PLoS ONE 6(8) : e23693. doi:10.1371/journal.pone.0023693
- Boistel, R., Aubin, T., Cloetens, P., Langer, M., Gillet, B., Josset, P., Pollet, N. and Herrel, A. (2011) Whispering to the Deaf : Communication by a Frog without External Vocal Sac or Tympanum in Noisy Environments. PLoS ONE 6(7) : e22080. doi:10.1371/journal.pone.0022080
- Sinzelle, L., Carradec, Q., Paillard, E., Bronchain, O.J. and Pollet, N. (2011) Characterization of a Xenopus tropicalis Endogenous Retrovirus with Developmental and Stress-Dependent Expression. J Virol 85:2167-2179.
- Pegoraro, C., Pollet, N. and Monsoro-Burq AH. (2011) Tissue-specific expression of Sarcoplasmic/Endoplasmic Reticulum Calcium ATPases (ATP2A/SERCA) 1, 2, 3 during Xenopus laevis development. Gene Expr Patterns 11:122-8.
2010
- Hellsten, U., Harland, R.M., Gilchrist, M.J., Hendrix, D., Jurka, ., Kapitonov, V., Ovcharenko, I., Putnam, N., Shu, S., Taher, L., Blitz, I., Blumberg, B., Dichann, D., Dubchak, I., Fletcher, R., Gerhard, D., Goodstein, D., Graves, T., Grigoriev, I., Grimwood, J., Kawashima, T., Lindquist, E., Mead, P., Mitros, T., Ota, Y., Poliakov, A., Pollet, N., Robert, J., Salamov, A., Sater, A., Schmutz, J., Terry, A., Vize, P., Warren, W., Wells, D., Wills, A., Zimmerman, L., Grainger, R., Grammer, T., Khokha, M., Richardson, P. and Rokhsar, D. (2010) The genome of the western clawed frog Xenopus tropicalis. Science 328:633-6.
- Pollet, N. (2010) Expression of immune genes during metamorphosis of Xenopus: a survey. Front Biosci. 15:348-58.
- Ymlahi-Ouazzani, Q., Bronchain, O.J., Paillard, E., Ballagny, C., Chesneau, A., Jadaud, A., Mazabraud, A. and Pollet, N. (2010) Reduced levels of survival motor neuron protein leads to aberrant motoneuron growth in a Xenopus model of muscular atrophy, Neurogenetics. 11 :27-40.
- Smelty, P., Marchal, C., Renard, R., Sinzelle, L., Pollet, N., Dunon, D., Jaffredo, T., Sire, J-Y, Fellah, J.S. (2010) Identification of the pre–T-cell receptor α chain in nonmammalian vertebrates challenges the structure–function of the molecule. Proc Natl Acad Sci U S A 107:19991-19996.
Jonathan, Filée
Permanent Researcher (CR1), CNRS.
Equipe POLYGNOME
Tèl. 33 1 69 82 37 05
Fax 33 1 69 82 37 36
Jonathan.Filee@egce.cnrs-gif.fr
Evolutionnary genomics of Viruses and Transposons
Viruses are ubiquitous components of the biosphere and their number exceeds that of cells by at least an order of magnitude. This abundance is also accompanied by an extraordinary diversity in genome size, composition and organisation and viruses have been divided into many different classes based on these criteria. These observations raise multiple questions about the origin and evolution of different viral classes.
My researches focus on the diversity and genome evolution of various classes of mobile elements including large DNA viruses and phages, Retroviruses/Retrotransposons and DNA transposons. I’m also interested by the evolutionary relationships between these elements and their cellular hosts i.e. lateral gene exchange and exaptation of viral genes to accomplish cellular processes.
Curriculum vitae
2000 : DEA « Biodiversité : Génétique, Histoire et Mécanisme de l’évolution », Université Paris XI Orsay (France).
2000-2003 : PhD degree, Université Paris XI Orsay (France), IGM (Patrick Forterre Lab).
2003-2007 : Postdoc, Université Toulouse III (France), LMGM (Henry Krisch and Mick Chandler Labs).
2007- : Permanent Researcher (CR), CNRS, LEGS
Publications
Filée J, Rouault JD, Harry M, Hua-Van A (2015) Mariner transposons are sailing in the genome of the blood-sucking bug Rhodnius prolixus. BMC genomics. 15;16(1):1061
Filée J. (2015) Genomic comparison of closely related Giant Viruses supports an accordion-like model of evolution. Front. Microbiol. 6:593.
Filée J. (2014) Multiple occurrences of Giant Virus core genes acquired by Eukaryotic genomes: the visible part of the iceberg ? Virology. 466,53-59
Kamoun C, Payen T, Hua-Van A, Filée J. (2013) Improving prokaryotic transposable elements identification using a combination of de novo and profile HMM methods. BMC Genomics 11;14:700
Filée J. (2013) Route of NCLDV evolution: the genomic accordion. Curr Opin Virol. 3(5):595-9
Metcalfe CJ, Filée J, Germon I, Joss J, Casane D. (2012) Mode and tempo in inflation of size of the Australian lungfish (Neoceratodus forsteri) genome: a major role for CR1-like elements. Mol. Biol. Evol. 9(11):3529-39
Hua-Van A, Le Rouzic A, Boutin TS, Filée J, Capy P. (2011) The struggle for life of the genome’s selfish architects. Biol. Direct 6:19..
Filée J, Chandler M. (2010) Gene exchange and the origin of Giant Viruses. Intervirology 53(5):354-61.
Rouault JD, Casse N, Chénais B, Hua-Van A, Filée J, Capy P (2009) Automatic classification within families of transposable elements: application to the mariner Family. Gene.15;448(2):227-32.
Filée J (2009) Lateral gene transfer, lineage-specific gene expansion and the evolution of Nucleo Cytoplasmic Large DNA viruses.J Invertebr Pathol. 101(3):169-71
Filée J, Pouget N, Chandler M. (2008) Phylogenetic evidence for extensive lateral acquisition of cellular genes by Nucleocytoplasmic Large DNA Viruses.BMC Evol Biol. 26;8:320
Filee J, Siguier P, Chandler M (2007) Insertion Sequence diversity in Archaea. Microbiol Mol Biol Rev. 71(1):121-57
Filee J, Siguier P, Chandler M (2007) I am what I eat and I eat what I am: acquisition of bacterial genes by Giant Viruses. Trends Genet. 23(1):10-5
Siguier P, Filee J, Chandler M (2006) Insertion Sequences in Prokaryotic Genomes. Curr. Opin. Microbiol. 9(5):526-31
Filee J, Bapteste E, Susko E, Krisch HM (2006) A Selective Barrier to Horizontal Gene Transfer in the T4-Type Bacteriophages that Has Preserved a Core Genome with the Viral Replication and Structural Genes. Mol. Biol. Evol. 23(9):1688-1696
Filee J, Comeau AM, Suttle CA, Krisch HM (2006) T4-type bacteriophages. Med Sci. 22(2):111-2.
Filee J, Tetart F, Suttle CA, Krisch HM (2005) Marine T4-type bacteriophages, a ubiquitous component of the dark Matter of the biosphere. Proc.Natl.Acad.Sci.USA. 102(35):12471-6
Filee J, Forterre P (2005) Viral proteins functioning in organelles: a cryptic origin? Trends Microbiol. 13(11):510-3
Filee J, Forterre P, Laurent J (2003) The role played by viruses on the Evolution of their cellular host: a view on informational proteins phylogenies. Res. Microbiol. 154:237-43
Myllykallio H, Leduc D, Filee J, Liebl U (2003) Life without dihydrofolate reductase FolA. Trends Microbiol. 11(5):220-3
Gadelle D, Filee J, Buhler C, Forterre P (2003) Phylogenomics of type II DNA topoisomerases. BioEssay 25: 232-242.
Myllykallio H, Lipowsky G, Leduc D, Filee J, Forterre P, Liebl U (2002) An alternative flavin-dependent mechanism for thymidylate synthesis. Science 5578:105-7.
Filee J, Forterre P, Sen-Lin T, Laurent J (2002) Evolution of DNA polymerase families: evidences for multiple gene exchange between cellular and viral proteins. J Mol Evol 54:763-73.